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Aug 10 - 15, 2018

First Croucher Summer Course In Precision Genome Engineering By CRISPR: Course Program

Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong (map)

Day 1 (10 August 2018 Friday)
Introduction to CRISPR
Time Event Venue
09:00 Opening remarks
(Prof. Dong-Yan Jin and Prof. Ben Berkhout)
SR1
09:15 Introduction to CRISPR
(Dr. Bo Gao)
10:30 Cutting-edge CRISPR technologies
(Dr. Alan Wong)
11:45 Bioinformatics of gRNA design
(Prof. Qi Liu)
13:00 Lunch Exh
14:30 Practical session 1
(Dr. Sam Yuen)
  1. Practical briefing
  2. gRNA design
  3. Transfection of CRISPR plasmids into HEK293 cells
Lab

 

Day 2 (11 August 2018 Saturday)
CRISPR in model systems
Time Event Venue
09:00 Genome-editing in preimplantation embryos and in EPSCs
(Prof. Pengtao Liu)
SR1
10:30 Organoids as a model system and their tools for genetic manipulation
(Dr. Bon-Kyoung Koo)
12:20 Lunch Exh
14:30 Practical Session 2
(Dr. Sam Yuen)
  1. Cloning of CRISPR construct
  2. Introduction to CRISPR library construction and other CRISPR resources
Lab

 

Day 3 (12 August 2018 Sunday)
Mechanism and delivery of CRISPR
Time Event Venue
09:00 Crystallography and HF-AFM of CRISPR-Cas9
(Prof. Osamu Nureki)
SR1
10:30 Novel CRISPR expression strategies
(Prof. Ben Berkhout and all invited speakers)
SR1
12:20 Lunch Exh
14:30 Practical Session 3
(Dr. Sam Yuen)
  1. Harvest of genomic DNA
  2. PCR verification of CRISPR editing
Lab

 

Day 4 (13 August 2018 Monday)
One-Day Symposium on Precision Genome Engineering
Time Event Venue
08:30 Opening remarks
(HKU representatives)
LT1
08:45 Bioinformatics of CRISPR editing
(Dr. Qi Liu)
09:30 Molecular mechanism of CRISPR-Cas and structure-guided development of genome-editing tools towards medical applications
(Prof. Osamu Nureki)
10:25 Genome-editing in preimplantation embryos and in EPSCs
(Prof. Pengtao Liu)
11:10 CRISPR/Cas-assisted genetics in intestinal organoids
(Dr. Bon-Kyoung Koo)
11:55 CRISPR-based genetic screens
(Dr. Kosuke Yusa)
12:40 Lunch Exh
14:00 Gene editing of the functional cancer genome
(Dr. Sidi Chen)
LT1
14:45 The use of large scale functional genomic screens for the identification of novel drug targets and effective combination therapies
(Dr. Roderick Beijersbergen)
15:40 CRISPR-Cas9 screening to identify cancer dependencies
(Dr. Andrew Aguirre)
16:25 CRISPR-Cas genome editing technologies for therapeutics
(Dr. Shengdar Tsai)
17:10 Anti-HIV CRISPR
(Prof. Ben Berkhout)

 

Day 5 (14 August 2018 Tuesday)
Time Event Venue
09:00 CRISPR-based genetic screens
(Dr. Kosuke Yusa)
SR1
10:15 CRISPR screens in vivo
(Dr. Sidi Chen)
11:30 Development and application of CRISPR/CAS9 technologies for large scale pooled screening
(Dr. Roderick Beijersbergen)
14:15 Free afternoon -
16:15 Hiking in Lamma Island (if weather permits) -

 

Day 6 (15 August 2018 Wednesday)
Time Event Venue
09:00 Discovery of therapeutic targets and drug resistance mechanisms by functional genetic screening
(Dr. Andrew Aguirre)
SR1
10:15 Defining the genome-wide activity of CRISPR-Cas nucleases for therapeutics
(Dr. Shengdar Tsai)
11:30 Workshop
(Prof. Ben Berkhout and all invited speakers)
12:45 Lunch Exh
14:25 Practical Session 4
(Dr. Sam Yuen)
  1. PCR verification and data analysis
  2. Single cell isolation
  3. Cell sorting demonstration
Lab
16:55 Concluding remarks
(Prof. Dong-Yan Jin and Prof. Ben Berkhout)
SR1

 

Venue Code
SR1 Seminar Room 1, G/F, Laboratory Block
Exh Exhibition Area, G/F, Laboratory Block
Lab LG-19, G/F, Laboratory Block
LT1 Lecture Theatre 1, G/F, William Mong Block
map

Last updated: July 20, 2018