School of Biomedical Sciences is pleased to invite you to join the following seminar:

Date: 5 December 2023 (Tuesday)
Time: 4:00 pm – 5:00 pm
Venue: Lecture Theatre 2, G/F, William M.W. Mong Block, 21 Sassoon Road

Speaker: Professor Daniel Hebenstreit, School of Life Sciences, The University of Warwick
Talk Title: Assembly dynamics of nuclear condensates are critical for transcriptional effects

Biography

Daniel Hebenstreit studied genetics and mathematics at the University of Salzburg in Austria. After a postdoctoral stay at the Laboratory of Molecular Biology in Cambridge, UK, he was appointed to a faculty position at the University of Warwick, we he has been researching transcription in mammalian cells since.

Abstract
There is a growing recognition that Liquid-Liquid Phase Separation (LLPS) is involved in many cellular processes, including transcription or chromatin organisation. However, the biological implications of LLPS are still largely unresolved.

We use an optogenetics system to induce in vivo condensation of an unstructured protein domain in the nucleus of a mammalian cell line. Microscopic analysis revealed three main condensate phenotypes, which we isolated by flow cytometric sorting.

Characterization of these by next generation sequencing techniques to capture chromatin topology and transcriptional dynamics, along with advanced imaging analysis, revealed formation and disassembly kinetics as the main determinants of their effects on transcription.
 

ALL ARE WELCOME

Should you have any enquiries, please feel free to contact Miss Angela Wong at 3917 9216.