School of Biomedical Sciences cordially invites you to join the following seminar:

Speaker: Dr. Jung-Chi Liao, Founder and CEO of Syncell
Talk Title: Microscopy-guided protein photo-biotinylation for high-precision spatial proteomic discovery

Date: 8 July 2024 (Monday)
Time: 11:00 am – 12:00 pm
Venue: Seminar Room 1, G/F, Laboratory Block, 21 Sassoon Road
Host: Professor Mu He (School of Biomedical Sciences) and Professor Wei Dai (Department of Clinical Oncology)

Biography

Dr. Jung-Chi Liao is the founder and CEO of Syncell, a spinoff company of Academia Sinica in Taiwan based on his pioneering Microscoop technology. Prior to Syncell, he was a Research Fellow/Associate Research Fellow at Academia Sinica and an Assistant Professor at Columbia University. In addition to the Microscoop technology, Dr. Liao was known for his contribution in superresolution microscopy for primary cilium studies, publishing key papers on resolving the protein complex structures of primary cilia. He received the Outstanding Research Award of Ministry of Science and Technology, Taiwan, and National Innovation Award in Taiwan. Dr. Liao was a Research Associate at Stanford University and a postdoc at University of California, Berkeley. He received his PhD from MIT.

Abstract

Unbiased microscopy-guided proteomics at single-organelle spatial scales is desired for revealing unknown protein constituents in specific disease- or functional-associated regions at the level of molecular-molecular interactions. Here, we achieve protein spatial purification by using Microscoop, a firmware-integrated microscopy platform that triggers in situ subcellular photo-biotinylation of proteins at user-defined regions of interest (ROIs), one field of view (FOV) at a time, for thousands of FOVs fully automatically. Sufficient proteins are biotinylated and pulled down from a cell, FFPE tissue, or fresh frozen tissue sample at 240-nm precision. Subsequent LC-MS/MS is implemented to reveal the subcellular proteome in high sensitivity, specificity, and resolution. Microscoop has been successfully used to identify novel protein constituents of stress granules, primary cilia, immune synapses, TDP-43 aggregates, amyloid β plagues, etc.

ALL ARE WELCOME
Should you have any enquiries, please feel free to contact Miss Crystal Chan at 3917 6830.