Dr. Xiubao Chang
President and Chief Scientist, TutumGene
Emeritus Consultant
Department of Biochemistry & Molecular Biology College of Medicine, Mayo Clinic in Arizona, USA

Date: Thursday, 10-May-2018
Time: 4:00 p.m.
Venue: Seminar Room 4, G/F, Laboratory Block,
Faculty of Medicine Building,
21 Sassoon Road, Pokfulam, Hong Kong

Summary:
Many disorders are caused by gene mutations. Such disease-causing mutations can potentially be corrected by homology-directed recombination (HDR). However, HDR is a complex processing of orchestrated reactions involving multiple factors. The greatest challenge in HDR is the creation of the recombinogenic DNA ends near the target site. Development of the nuclease-based technologies, such as CRISPR/Cas9 provide powerful tools to generate double strand DNA (dsDNA) break (DSB) near the target site; however, a safer genome editing technology is needed. In our novel approach, I.e., ssDNA (single strand DNA)-RecA-CPP (cell penetrating peptide), the system does not need to generate a DSB near the target site, Cas9 or any other nucleases are not needed, thus, preventing the mutations introduced by DSBs

ALL ARE WELCOME