Dr. Kedar Natarajan
Danish-Institute of Advanced Study (D-IAS), Functional Genomics and Metabolism Unit
University of Southern Denmark, Odense, Denmark

Date: Monday, 22-October-2018
Time: 4:00 p.m.
Venue: Seminar Room 1, G/F, Laboratory Block,
Faculty of Medicine Building,
21 Sassoon Road, Pokfulam, Hong Kong

Summary:
Pluripotent embryonic stem cells (ESCs) have a unique cell cycle and gene expression program that facilitates lineage determination alongside cellular scaling during development. Dr. Natarajan’s team generate FUCCI-mESCs that capture spatio-temporal cell cycle progression at the singlecell level. Here Dr. Natarajan presents their work investigating cell cycle dynamics combining flow cytometry (FACS) and RNA sequencing (scRNA-seq) of ~700 single ESCs throughout entire cell cycle and across two culture conditions. They integrate single-cell measurements and assess how cellular features affect gene dynamics during cell cycle, reconstruct single-cell gene expression profiles and predict gene contribution to cell cycle, stem cell fate and in maintaining cellular homeostasis.

ALL ARE WELCOME