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Dec 10, 2018

Seminar - Quantitative proteomics as tool to study transcription factor DNA interactions (Speaker: Dr. Mårten Larsson)

Dr. Mårten Larsson
Department of Medical Biochemistry and Microbiology
Uppsala University, Uppsala, Sweden

Date: Monday, 10-December-2018
Time: 3:00 p.m.
Venue: Seminar Room 5, LG1/F, Laboratory Block, Faculty of Medicine Building
21 Sassoon Road, Pokfulam, Hong Kong​

Mass spectrometry-based proteomics includes methods mainly performed in a platform setting, due to the high cost of instrument maintenance. Therefore, the knowledge of quantitative mass spectrometry methods, instrument limitations and result interpretations can often be hard to acquire for the individual researcher. At the same time, sample preparation, mostly performed by the researcher himself, is the single most important step for achieving useful results in a proteomics experiment. For that reason, it is of outmost importance for the researcher to obtain the background needed to setup a successful mass spectrometry-based proteomics experiment. In this presentation, the speaker will in a simple way try to explain the basics of the mass spectrometer, important considerations during sample preparation, certain pitfalls in results interpretation, and present a few current quantitative proteomics methods. The talk fits the student or researcher with no or little previous knowledge in mass spectrometry-based proteomics and its methods.