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May 29, 2019

Seminar - Systematic Analysis of Differential Transcription Factor Binding to Non-Coding Variants in the Human Genome (Speaker: Dr. Jian Yan)

Dr. Jian Yan
Assistant Professor, Department of Biomedical Sciences
City University of Hong Kong

Date: Wednesday, 29-May-2019
Time: 11:00 a.m.
Venue: Seminar Room 1, G/F, Laboratory Block
Faculty of Medicine Building
21 Sassoon Road, Pokfulam, Hong Kong

Large numbers of sequence variants have been linked to complex human traits and diseases, but deciphering their biological function remains challenging especially for the non-protein-coding variants. Dr. Yan’s group have systematically assessed the differential binding of transcription factors (TF) to different alleles of non-coding variants in the human genome. Using an ultra-high throughput multiplex protein-DNA binding assay, they examined the binding of 270 human TFs to 95,886 common sequence variants associated with type 2 diabetes. They then employed a machine-learning approach to predict differential DNA binding of 124 TFs to other common non-coding variants in the human genome. The newly derived models outperformed current position-weight matrices (PWM) in describing TF binding to non-coding variants, and facilitated discovery of potential causal variants and dysregulated molecular pathways in human diseases.